A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms

作者：Yi, Li^*; Wu, Ting; Luo, Wenyuan; Zhou, Wen; Wu, Jun

来源：Neural Regeneration Research, 2014, 9(1): 69-75.

DOI：10.4103/1673-5374.125332

• nerve regeneration
• neurodegeneration
• late-onset Alzheimer's disease
• apolipoprotein E gene
• real-time PCR
• DNA sequencing
• risk factor
• allele
• neural regeneration

The apolipoprotein E gene epsilon 4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer's disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E epsilon 4 allele in neural regeneration in late-onset Alzheimer's disease cases.

• 出版日期2014-1
• 单位北京大学