ESCs Require PRC2 to Direct the Successful Reprogramming of Differentiated Cells toward Pluripotency

作者：Pereira Carlos F; Piccolo Francesco M; Tsubouchi Tomomi; Sauer Stephan; Ryan Natalie K; Bruno Ludovica; Landeira David; Santos Joana; Banito Ana; Gil Jesus; Koseki Haruhiko; Merkenschlager Matthias; Fisher Amanda G^*

来源：Cell Stem Cell, 2010, 6(6): 547-556.

DOI：10.1016/j.stem.2010.04.013

Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2 activity in PRC2-deficient ESCs. Surprisingly, the failure of PRC2-deficient ESCs to reprogram somatic cells is functionally dominant, demonstrating a critical requirement for PcG proteins in the chromatin-remodeling events required for the direct conversion of differentiated cells toward pluripotency.

• 出版日期2010-6-4