Shepherd's Purse Polyphenols Exert Its Anti-Inflammatory and Antioxidative Effects Associated with Suppressing MAPK and NF-κB Pathways and Heme Oxygenase-1 Activation

作者：Peng, Jinming; Hu, Tianyong; Li, Jin; Du, Jing; Zhu, Kerui; Cheng, Baohui; Li, Kaikai^*

来源：Oxidative Medicine and Cellular Longevity, 2019, 2019: 7202695.

DOI：10.1155/2019/7202695

• CAPSELLA-BURSA-PASTORIS
• CHEMICAL-COMPOSITION
• PLANT-EXTRACTS
• IN-VITRO
• INFLAMMATION
• INHIBITION
• MACROPHAGES
• EXPRESSION
• MICE
• IDENTIFICATION

Shepherd's purse (Capsella bursa-pastoris (L.) Medik.), a wild herb as a traditional herbal medicine, has been proved with multiple healthy benefits. In this study, the chemical constituents of shepherd's purse were identified by UPLC-QTOF-MS/MS. The antioxidative and anti-inflammatory potential of shepherd's purse extract (SPE) were also investigated applying lipopolysaccharide- (LPS-) induced inflammation in RAW 264.7 macrophages and a carrageenan-induced mice paw edema model. Twenty-four chemical compounds were identified mainly including phenolic acids and flavonoids. The data also indicated SPE inhibited the productions of NO, PGE(2), TNF-alpha, and IL-6 stimulated with LPS. In addition, SPE inhibited the increase of reactive oxygen species (ROS) and upregulated the expression of heme oxygenase-1 (HO-1). We further found that SPE inhibited the phosphorylation of P38 MAPK and activation of NF-kappa B. In vivo mice model also indicated that SPE showed strong antioxidative and anti-inflammatory activity.

• 出版日期2019
• 单位华中农业大学