A standardized toolkit for genetic engineering of CTG Glade yeasts

作者:Defosse Tatiana A.; Courdavault Vincent; Coste Alix T.; Clastre Marc; de Bernonville Thomas Duge; Godon Charlotte; Vandeputte Patrick; Lanoue Arnaud; Touze Antoine; Linder Tomas; Droby Samir; Rosa Carlos A.; Sanglard Dominique; d'Enfert Christophe; Bouchara Jean-Philippe; Giglioli-Guivarc'h Nathalie; Papon Nicolas*
来源:Journal of Microbiological Methods, 2018, 144: 152-156.

摘要

We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG Glade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.

  • 出版日期2018-1