A new tool for detection of extracellular traps

作者：Proust Alize; Levesque Julie Christine; Barat Corinne; Sato Sachiko; Tremblay Michel J^*

来源：Methods and Applications in Fluorescence, 2018, 6(3): 037002.

DOI：10.1088/2050-6120/aac51b

• extracellular traps
• fluorescence microscopy
• nuclear DNA
• extracellular DNA

Extracellular traps ejected by various immune cells (neutrophils, macrophages, eosinophils and mast cells) have several immune functions, either protective against pathogens or deleterious in some autoimmune or inflammatory disorders. Since their first description in 2004, the mechanisms of extracellular traps formation have been extensively investigated though still not fully understood. We describe here a new tool for the detection of extracellular traps by fluorescence microscopy in a single-step staining protocol, which does not require any wash. The approach uses the GreenGlo (TM) DNAdye, which can differentiate between nuclear DNA and extracellular DNA(extracellular traps) released from cells using different fluorescence excitation wavelengths. GreenGlo (TM) staining is suitable for adherent and non-adherent cells and is expected to be extendable to extracellular traps from other cells types (i.e. eosinophils, mast cells and monocytes).

• 出版日期2018-6