Toll-Like Receptor 6 and Connective Tissue Growth Factor Are Significantly Upregulated in Mitomycin-C-Treated Urothelial Carcinoma Cells Under Hydrostatic Pressure Stimulation

作者：Chen Shao Kuan; Chung Chih Ang; Cheng Yu Che; Huang Chi Jung; Chen Wen Yih; Ruaan Ruoh Chyu; Li Chuan; Tsao Chia Wen; Hu Wei Wen; Chien Chih Cheng^*

来源：Genetic Testing and Molecular Biomarkers, 2014, 18(6): 410-416.

DOI：10.1089/gtmb.2013.0443

Background: Urothelial carcinoma (UC) is the most common histologic subtype of bladder cancer. The administration of mitomycin C (MMC) into the bladder after transurethral resection of the bladder tumor (TURBT) is a common treatment strategy for preventing recurrence after surgery. We previously applied hydrostatic pressure combined with MMC in UC cells and found that hydrostatic pressure synergistically enhanced MMC-induced UC cell apoptosis through the Fas/FasL pathways. To understand the alteration of gene expressions in UC cells caused by hydrostatic pressure and MMC, oligonucleotide microarray was used to explore all the differentially expressed genes. Results: After bioinformatics analysis and gene annotation, Toll-like receptor 6 (TLR6) and connective tissue growth factor (CTGF) showed significant upregulation among altered genes, and their gene and protein expressions with each treatment of UC cells were validated by quantitative real-time PCR and immunoblotting. Conclusion: Under treatment with MMC and hydrostatic pressure, UC cells showed increasing apoptosis using extrinsic pathways through upregulation of TLR6 and CTGF.

• 出版日期2014-6
• 单位中央民族大学