Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1 beta: Evidence for opioid-immune crosstalk

作者：Mohan Shekher; Davis Randall L; DeSilva Udaya; Stevens Craig W^*

来源：Journal of Neuroimmunology, 2010, 227(1-2): 26-34.

DOI：10.1016/j.jneuroim.2010.06.007

• Interleukin-1 beta
• Morphine
• Mu opioid receptor
• SK-N-SH cells
• Opioid-immune crosstalk

Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1 beta) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1 beta increased it, as assessed by real-time quantitative PCR These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1 beta-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1 beta and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

• 出版日期2010-10-8