科研之友
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摘要
Utilising the affinity and high combination ability between silver nanocubes and amino group (-NH2), a novel electrochemical immunosensor was constructed for the ultrasensitive detection of microcystin-LR (MC-LR) based on G4-polyamidoamine (PAMAM) dendrimer and Ag nanocubes as immobilised substrate of anti-MC-LR. G4-PAMAM dendrimers were covalently bound on the chitosan (CHIT) - modified electrode by glutaraldehyde (GA), providing abundant amino groups to absorb much more Ag nanocubes comparing without using PAMAM. Subsequently, antibodies of MC-LR were immobilised with highly dense through Ag-NH2. K3Fe(CN)(6)/K4Fe(CN)(6) was used as electroactive redox probe. Ag nanocubes/PAMAM can enhance the antibody loading amount, which would bind more MC-LR and hinder the electron transfer of K3Fe(CN)(6)/K4Fe(CN)(6). Differential pulse voltammetry (DPV) was employed to evaluate the analytical performance of the fabricated signal-off immunosensor. The response current had negative correlation with the concentration of MC-LR. The linear range covered was from 0.05ng/mL to 25 mu g/mL with detection limit (DL) of 0.017ng/mL at 3 sigma. The proposed approach showed high specificity for the detection of MC-LR, with acceptable reproducibility, stability and reliability. Compared with the enzyme-linked immunoassay (ELISA) method by analyzing real water samples from Dian Lake, this immunosensor revealed acceptable accuracy with a relative error of 12.7%, indicating a potential alternative method for MC-LR detection in water sample.
- 出版日期2016-2-19
- 单位云南省环境科学研究所; 云南师范大学
