Microfluidic inverse phase ELISA via manipulation of magnetic beads

作者：Chen Hong; Abolmatty Assem; Faghri Mohammad^*

来源：Microfluidics and Nanofluidics, 2011, 10(3): 593-605.

DOI：10.1007/s10404-010-0692-2

• Microfluidics
• ELISA
• Lab-on-a-chip
• Magnetic beads
• Capillary force

We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are pre-encapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (mu IPELISA). mu IPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use mu IPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng mu l(-1) of digoxigenin-labeled amplicon, mu IPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct mu IPELISA in two parallel microchannels.

• 出版日期2011-3