Analysis of Serum Cytokine Levels in Larynx Squamous Cell Carcinoma and Dysplasia Patients

作者：Eyigor Mete^*; Eyigor Hulya; Osma Ustun; Yilmaz MustafaDeniz; Erin Nuray; Selcuk Omer Tarik; Sezer Cem; Gultekin Meral; Koksoy Sadi

来源：Iranian Journal of Immunology, 2014, 11(4): 259-268.

• Bead Array
• Cytokine
• Dysplasia
• HNSCC
• Larynxm Carcinoma

Background: Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation. Objective: To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls. Methods: Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-alpha (TNF-alpha) and Interferon-gamma (IFN-gamma) levels. Additionally, IL-8 levels were determined through ELISA. Results: IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05). Conclusion: Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.

• 出版日期2014-12