An iminofluorescein-Cu2+ ensemble probe for selective detection of thiols

作者：Wang, Huilin; Zhou, Guodong; Chen, Xiaoqiang^*

来源：Sensors and Actuators B: Chemical , 2013, 176: 698-703.

DOI：10.1016/j.snb.2012.10.006

• Iminofluorescein-Cu2+ ensemble
• Fluorescence
• Sensor
• Thiols
• Biological imaging

An iminofluorescein-Cu2+ ensemble probe was prepared for the detection of thiols in aqueous solution. In HEPES buffer, among various amino acids, only cysteine (Cys) induced fluorescence enhancement of Cu2+ ensemble. The mechanism was proposed that addition of Cys induced decomplexation of Cu2+ from weak-fluorescent ensemble, followed by hydrolytic cleavage of the resulted Schiff base to give a strongly fluorescent fluoresceinaldehyde. By fluorescence titration using 2 mu M iminofluorescein-Cu2+ ensemble probe, the detection limit for Cys was evaluated to be 9 mu M with a signal-to-noise ratio of 3. Furthermore, the confocal microscopy experiments demonstrated that this sensor was potential for the imaging of thiols in living cells.

• 出版日期2013-1
• 单位南京工业大学; 材料化学工程国家重点实验室