A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies

作者：Liu, Xiaohui; Lu, Tingting; Yu, Shuliang; Li, Ying; Huang, Yuchen; Huang, Tao; Zhang, Lei; Zhu, Jingjie; Zhao, Qiang; Fan, Danlin; Mu, Jie; Shangguan, Yingying; Feng, Qi; Guan, Jianping; Ying, Kai; Zhang, Yu; Lin, Zhixin; Sun, Zongxiu; Qian, Qian; Lu, Yuping; Han, Bin^*

来源：Plant Molecular Biology, 2007, 65(4): 403-415.

DOI：10.1007/s11103-007-9174-7

• comparative analysis
• full-length cDNA
• Indica and japonica rice
• Oryza sativa
• transcriptome

Relatively few indica rice full-length cDNAs were available to aid in the annotation of rice genes. The data presented here described the sequencing and analysis of 10,096 full-length cDNAs from Oryza sativa subspecies indica Guangluai 4. Of them, 9,029 matched rice genomic sequences in publicly-available databases, and 1,200 were identified as new rice genes. Comparison with the knowledge-based Oryza Molecular Biological Encyclopedia japonica cDNA collection indicated that 3,316 (41.6%) of the 7,965 indica-japonica cDNA pairs showed no distinct variations at protein level (2,117 indica-japonica cDNA pairs showed fully identical and 1,199 indica-japonica cDNA pairs showed no frame shift). Moreover, 3,645 (45.8%) of the indica-japonica pairs showed substantial differences at the protein level due to single nucleotide polymorphisms (SNPs), insertions or deletions, and sequence-segment variations between indica and japonica subspecies. Further experimental verifications using PCR screening and quantitative reverse transcriptional PCR revealed unique transcripts for indica subspecies. Comparative analysis also showed that most of rice genes were evolved under purifying selection. These variations might distinguish the phenotypic changes of the two cultivated rice subspecies indica and japonica. Analysis of these cDNAs extends known rice genes and identifies new ones in rice.

• 出版日期2007-11
• 单位上海交通大学; 中国科学院上海生命科学研究院; 中国农业科学院; 复旦大学