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摘要
BACKGROUNDUrine cytology has been used for screening of bladder cancer but has been limited by its low sensitivity. UroVysion is a multiprobe fluorescence in situ hybridization (FISH) assay that detects common chromosome abnormalities in bladder cancers. For this study, the authors evaluated the effectiveness of multiprobe FISH and urine cytology in detecting urothelial cell carcinoma (UCC) in the same urine sample. %26lt;br%26gt;METHODSIn total, 1835 cases with the following criteria were selected: valid results from both the multiprobe FISH assay and urine cytology in the same urine sample, histologic and/or cystoscopic follow-up within 4 months of the original tests, or at least 3 years of clinical follow-up information. The results of FISH and cytology were correlated with clinical outcomes derived from a combination of histologic, cystoscopic, and clinical follow-up information. %26lt;br%26gt;RESULTSOf 1835 cases, 1045 cases were from patients undergoing surveillance of recurrent UCC, and 790 were for hematuria. The overall sensitivity, specificity, positive predictive value, and negative predictive value in detecting UCC were 61.9%, 89.7%, 53.9%, and 92.4%, respectively, for FISH and 29.1%, 96.9%, 64.4%, and 87.5%, respectively, for cytology. The performance of both FISH and cytology generally was better in the surveillance population and in samples with high-grade UCC. In 95 of 296 cases with atypical cytology that were proven to have UCC, 61 cases, mostly high-grade UCC, were positive using the multiprobe FISH assay. %26lt;br%26gt;CONCLUSIONSThe UroVysion multiprobe FISH assay was more sensitive than urine cytology in detecting UCC, but it produced more false-positive results. The current data suggest that the use of FISH as a reflex test after an equivocal cytologic diagnosis may play an effective role in detecting UCC. Cancer (Cancer Cytopathol) 2013;121:591-597. (c) 2013 American Cancer Society. %26lt;br%26gt;The authors evaluate the UroVysion multiprobe fluorescence in situ hybridization (FISH) assay versus urine cytology for detecting urothelial cell carcinoma (UCC) from 1835 concurrent urine samples. The results reveal higher sensitivity but decreased specificity with the multiprobe FISH assay compared with cytology, suggesting that it may be efficient to use the FISH assay for patients at high risk for UCC, like those who have cytologic atypia.
- 出版日期2013-10
