Comparison of anti-inflammatory mechanisms of mango (Mangifera Indica L.) and pomegranate (Punica Granatum L.) in a preclinical model of colitis

作者：Kim Hyemee; Banerjee Nivedita; Ivanov Ivan; Pfent Catherine M; Prudhomme Kalan R; Bisson William H; Dashwood Roderick H; Talcott Stephen T; Mertens Talcott Susanne U^*

来源：Molecular Nutrition & Food Research, 2016, 60(9): 1912-1923.

DOI：10.1002/mnfr.201501008

• Colitis Mango mTOR pathway Pomegranate Rat

Scope: Tannin-rich fruits have been evaluated as alternative prevention strategies for colorectal cancer based on their anti-inflammatory properties. This study compared tannin-rich preparations from mango (rich in gallotannins) and pomegranate (rich in ellagitannins) in the dextran sodium sulfate-induced colitis model.
Methods and results: In rats, mango and pomegranate beverages decreased intestinal inflammation and the levels of pro-inflammatory cytokines inmucosa and serum. Themango beverage suppressed the ratio of phosphorylated/total protein expression of the IGF-1R-AKT/mTOR axis and downregulated mRNA expression of Igf1, Insr, and pik3cv. Pomegranate decreased p70S6K and RPS6, as well as Rps6ka2, Map2k2, and Mapk1 mRNA. In silico modeling indicated a high binding of docked of gallic acid to the catalytic domain of IGF-1R, which may suppress the activity of the enzyme. Ellagic acid docked effectively into the catalytic domains of both IGF-1R and EGFR. In vitro assays with lipopolysaccharide-treated CCD-18Co cells using polyphenolic extracts from each beverage, as well as pure compounds, corroborated the predictions made in silico.
Conclusion: Mango polyphenols inhibited the IGF-1R-AKT/mTOR axis, and pomegranate polyphenols downregulate the mTOR downstream pathway through reductions in ERK1/2. These results suggest that extracts rich in gallo-and ellagitannins act on different molecular targets in the protection against ulcerative colitis.

• 出版日期2016-9