Dual-Label Chemiluminescence Strategy for Multiplexed Immunoassay of 20 Fluoroquinolones, 15 beta-Lactams, 15 Sulfonamides, and CAP in Milk

作者：Tao, Xiaoqi^*; Wang, Jia; Xie, Yuejie; Zuo, Xiaowei; Mo, Fang; Zhou, Song; Li, Hongjun

来源：Food Analytical Methods, 2017, 10(9): 3009-3022.

DOI：10.1007/s12161-017-0865-7

• Chemiluminescence
• Multiplexed immunoassay
• Fluoroquinolones
• beta-Lactams
• Sulfonamides
• Chloramphenicol

In this paper, a novel dual-label time-resolved chemiluminescent multiplexed immunoassay (DLTRC-MIA) based on the distinction of the kinetic characteristics of horseradish peroxidase (HRP) and alkaline phosphatase (ALP) with approximate estimation approach for simultaneous determination of 20 fluoroquinolones (FQs), 15 beta-lactams, 15 sulfonamides (SAs), and chloramphenicol (CAP) in milk was developed. The strategy integrated a single-chain variable fragment-alkaline phosphatase fusion protein (scFv-ALP), a recombinant penicillin-binding protein (PBP) 2x*, a monoclonal antibody (MAb), and a polyclonal antibody (PAb) in one immunoassay and in a single well together to fulfill the simultaneous detection of 51 low-molecular weight contaminants (20 FQs, 15 beta-lactams, 15 SAs, and CAP). The limits of detection for FQs, beta-lactams, SAs, and CAP range from 0.29 mu g L-1 for ciprofloxacin (CIP) to 81.6 mu g L-1 for trovafloxacin (TRO), 0.27 mu g L-1 for ceftiofur (CEF) to 44.1 mu g L-1 for cephalexin (CEL), 0.089 mu g L-1 for sulfadimethoxine (SDM) to 2.7 mu g L-1 for sulfadiazine (SDZ), and 0.028 mu g L-1 for CAP, respectively. The results demonstrated that the detection limits of DLTRC-MIA meet the requirement of detection levels for 51 drug residues in milk, suitable for high-throughput screening of low-molecular weight contaminants.

• 出版日期2017-9
• 单位西南大学