Alteration of the expression and activation of tomato invertases during Botrytis cinerea infection

作者：Hyun Tae Kyung; Eom Seung Hee; Rim Yeonggil; Kim Ju Sung^*

来源：Plant Omics, 2011, 4(7): 413-417.

• Vacuolar invertase
• Extracellular invertase
• Botrytis cinerea
• In-silico analysis

Invertase is a key enzyme that hydrolyzes sucrose into glucose and fructose. In higher plants, invertases occur as a set of isoenzymes with different biochemical properties and sub-cellular locations. Although the expression and activity of invertase are known to be up-regulated when plants are exposed to stress-related stimuli, the interaction between the invertases and the necrotrophic pathogen Botrytis cinerea is not yet fully understood. Therefore, we have analyzed the expression pattern and activation of tomato invertases during an infection of B. cinerea. The expression of Lin5 and Lin6 is up-regulated during a response to B. cinerea, whereas other isoenzymes such as Lin7 and Lin8 are not significantly changed by B. cinerea in mature green fruit. In addition, the infection of B. cinerea results in increasing activity of vacuole and extracellular invertases. When the detached leaves were treated with methyl-jasmonic acid, the increased activities of vacuole and extracellular invertases were observed, and salicylic acid had no influence on the invertases activation. These findings indicate that carbohydrate partitioning via the activation of invertases might be involved in jasmonic acid-dependent defense responses during B. cinerea infections.

• 出版日期2011-12