Amyloid-like assembly of the low complexity domain of yeast Nab3

作者：O'Rourke Thomas W; Loya Travis J; Head PamelaSara E; Horton John R; Reines Daniel^*

来源：Prion, 2015, 9(1): 34-47.

DOI：10.1080/19336896.2014.997618

• CTD, carboxy terminal domain
• hnRNP, heterogeneous nuclear ribonucleoprotein
• A beta, amyloid beta
• SDD-AGE, semi-denaturing detergent agarose gel electrophoresis
• SDS, sodium dodecyl sulfate
• DMSO, dimethyl sulfoxide
• EDTA, ethylenediaminetetraacetic acid
• DHFR, dihydrofolate reductase
• PCR, polymerase chain reaction
• IPTG, isopropyl beta-D-1-thiogalactopyranoside
• BSA, bovine serum albumin
• CPEB, cytoplasmic polyadenylation element binding protein
• TEV, tobacco etch virus

Termination of transcription of short non-coding RNAs is carried out in yeast by the Nab3-Nrd1-Sen1 complex. Nab3 and Nrd1 are hnRNP-like proteins that dimerize and bind RNA with sequence specificity. We show here that an essential region of Nab3 that is predicted to be prion-like based upon its sequence bias, formed amyloid-like filaments. A similar region from Nrd1 also assembled into filaments in vitro. The purified Nab3 domain formed a macroscopic gel whose lattice organization was observed by X-ray fiber diffraction. Filaments were resistant to dissociation in anionic detergent, bound the fluorescent dye thioflavin T, and showed a beta-sheet rich structure by circular dichroism spectroscopy, similar to human amyloid beta which served as a reference amyloid. A version of the Nab3 domain with a mutation that impairs its termination function, also formed fibers as observed by electron microscopy. Using a protein fragment interaction assay, the purified Nab3 domain was seen to interact with itself in living yeast. A similar observation was made for full length Nab3. These results suggest that the Nab3 and Nrd1 RNA-binding proteins can attain a complex polymeric form and raise the possibility that this property is important for organizing their functional state during termination. These findings are congruent with recent work showing that RNA binding proteins with low complexity domains form a dynamic subcellular matrix in which RNA metabolism takes place but can also aberrantly yield pathological aggregated particles.

• 出版日期2015-1-2