A process for production of trehalose by recombinant trehalose synthase and its purification

作者：Liu, HongLing; Yang, ShaoJie; Liu, Qiang; Wang, Ruiming; Wang, Tengfei^*

来源：Enzyme and Microbial Technology, 2018, 113: 83-90.

DOI：10.1016/j.enzmictec.2017.11.008

• Trehalose synthase
• Trehalose
• Production
• Purification

The process for production of trehalose using trehalose synthase (TreS) to convert maltose into trehalose in one step is highly desirable in the industry. Nonetheless, the studies on industrial-scale production of trehalose by recombinant TreS in Escherichia coil are still scarce. In this study, a TreS from Pseudomonas putida ATCC47054 was expressed in E. colt BL21(DE3) via plasmids pET15b and pET22b. pET15b-treS showed better plasmid stability and TreS expression, which revealed that the highest activity, 39866 +/- 1420 U/(g dry cell weight) at the final lactose concentration of 4 g/L for 7 h at 27 degrees C in a 5-L fermentor at pH 8.0. The use of 30% (w/v) high maltose syrup as a substrate can extend the temperature tolerance of TreS to 60 degrees C. More than 64% of maltose can be converted into trehalose by adding 200 U of TreS per gram of maltose at 50 degrees C for 24 h. The total sugar content of the trehalose syrup reached 95.0% +/- 1.0% (w/w) after separation. The recovery rate of trehalose dehydrate reached 57.0% +/- 2.0% after slow cooling, and the purity was 99.0 +/- 0.2%. Our study revealed a safe and reliable process of trehalose production by recombinant TreS.

• 出版日期2018-6
• 单位天津科技大学