An omics based assessment of cadmium toxicity in the green alga Chlamydomonas reinhardtii

作者：Jamers An; Blust Ronny; De Coen Wim; Griffin Julian L; Jones Oliver A H^*

来源：Aquatic Toxicology, 2013, 126: 355-364.

DOI：10.1016/j.aquatox.2012.09.007

• Cadmium
• Metabonomics
• Oxidative stress
• Systems toxicology

The effects of cadmium were assessed in the freshwater alga Chlamydomonas reinhardtii. Algae were exposed to concentrations of 0, 8.1 or 114.8 mu M of cadmium and growth rates, gene transcription and metabolite profiles were examined after 48 and 72 h of exposure. In algae exposed to 8.1 mu M Cd, several genes were differentially transcribed after 48 h but no adverse growth related effects were detected. A transient effect on both gene transcription patterns and metabolite profiles could be discerned after 48 h of exposure but the majority of these changes disappeared after 72 h. In contrast, all effects were more pronounced at the 114.8 mu M cadmium exposure. Here growth was clearly reduced and transcription of a large number of genes involved in oxidative stress defense mechanisms was differentially increased. Metabolites involved in the glutathione synthesis pathway (an important antioxidant defense) were also affected but the effects of cadmium were found to be more pronounced at the transcript level than in the metabolome, suggesting that the former exhibits greater sensitivity toward cadmium exposure.

• 出版日期2013-1-15