Analysis and simulation of batch affinity processes applied to separation of biomolecules

作者：Guerrero German Patricia; Lucero Acuna Armando; Ma Montesinos Rosa; Tejeda Mansir Armando^*

来源：Journal of the Mexican Chemical Society, 2007, 51(2): 59-66.

• mathematical modeling
• batch affinity chromatography
• biomolecules

The scale-up and optimization of large-scale affinity chromatographic operations is of major industrial importance. In this work, a transport model which includes pore diffusion, external film resistance, and finite kinetic rate, was used to mathematically describe the performance of a batch affinity adsorption system. Experimental data from literature describing the adsorption of beta-galactosidase onto anti-beta-galactosidase immobilized on porous silica was used as a model system. The mathematical model was solved using the numerical method of lines (MOL) in a MATLAB platform. The use of the transport model is a unique way to predict batch affinity performance as well as to obtain a better understanding of the fundamental mechanisms involved in the bioseparations.

• 出版日期2007-6