Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins

作者：Arneth Borros M^*

来源：PeerJ, 2018, 6: e4462.

DOI：10.7717/peerj.4462

• Regulatory T cells
• CD4+T helper cells
• Lymphocytes
• CD8+cytotoxic T cells
• Interferon-gamma

This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immune-system response, on the intracellular. expression of extracellular activation markers on andcytokines T lymphocytes, and on the number of regulatory T cells (T-reg cells) were investigated via flow cytometry. Incubation with p-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which are cytoplasmic proteins, increased the expression of both extra cellular activation markers (CD69 and HLA-DR) and intracellular cytokines but did not significantly affect the number of T-reg cells. In contrast, incubation with human albumin or insulin, which are serum proteins, reduced both extracellular activation markers and intracellular cytokine expression and subsequently increased the number of T-reg cells. These findings may help to explain the etiological basis of autoimmune diseases.

• 出版日期2018-3-9