Association of Ozone with 5-Fluorouracil and Cisplatin in Regulation of Human Colon Cancer Cell Viability: In Vitro Anti-Inflammatory Properties of Ozone in Colon Cancer Cells Exposed to Lipopolysaccharides

作者：Simonetti V^*; Quagliariello V; Giustetto P; Franzini M; Iaffaioli R V

来源：Evidence-Based Complementary and Alternative Medicine, 2017, 2017: 7414083.

DOI：10.1155/2017/7414083

Introduction. Ozone therapy is an effective medical treatment for different diseases like mucositis, psoriasis, acute pain, neurovascular diseases, and cancer. The aim of this study is based on the association of different ozone concentration with 5-fluorouracil and cisplatin in human colon cancer cell (HT29 cell line) in order to investigate possible anticancer synergistic effects. Methods. HT29 cells were incubated with ozone at different concentration ranging from 10 up to 50 mu g/ml at different incubation time alone or in combination with cisplatin and 5-fluorouracil. Cell viability was performed by using a modified MTT method. Antiinflammatory studieswere conducted incubating HT29 with or without 20, 30, or 50 mu g/ml of ozone before exposure to lipopolysaccharides. Results. Ozone alone has a time and concentration dependent cytotoxicity against HT29 cells (IC50 at 24 h: 30 mu g/ml). Association of ozone with drugs increases cytotoxicity by 15-20%. Preincubation of ozone at 50 mu g/ml decreases IL-8, IL-6, and IL-1 beta production by 50, 56, and 70%, respectively, compared to untreated cells. Conclusion. These results indicated that ozone could be useful in colon cancer management in combination with 5-fluorouracil and cisplatin with significant inhibition of cytokines having a central role in colon cancer cell survival and chemoresistance.

• 出版日期2017