An acute reversible experimental model of pneumonia in pigs: time-related histological and clinical signs changes

作者：Villarino N; Garcia Tapia D; Lesman S; Lucas M; Robinson J; Brown S A; Martin Jimenez T^*

来源：Journal of Veterinary Pharmacology and Therapeutics, 2013, 36(3): 241-247.

DOI：10.1111/j.1365-2885.2012.01415.x

The objective of this study was to evaluate the long-term survival rates, clinical response, and lung gross and microscopic changes in pigs treated intratracheally with lipopolysaccharide of Escherichia coli 0111:B4 (LPS-Ec). Healthy pigs were randomly allocated to three groups: (i) no-LPS-Ec (n=1), (ii) LPS-Ec-T1 (1mg/mL, 10mL/pig) (n=7), and (iii) LPS-Ec-T2 (0.5mg/mL, 10mL/pig) (n=6). Two pigs from each dose group were euthanized at 24 (n=3 for T1), 48 and 144h post-LPS-Ec challenge. LPS-Ec-treated animals showed macroscopic lesions in middle lobes of the lung. A reversible recruitment of macrophages and neutrophils was observed at 24, 48, and 144h post-LPS-Ec challenge. The highest cellular infiltration level was observed at 24h after challenge. The highest clinical scores were evident in both experimental dose levels within 3 and 5h after LPS-Ec administration. Administration of LPS-Ec, under the conditions evaluated, can be used to induce a reproducible model of acute pulmonary inflammation in pigs.

• 出版日期2013-6