Apis mellifera haemocytes in-vitro: What type of cells are they? Functional analysis before and after pupal metamorphosis

作者：Negri Pedro^*; Maggi Matias; Szawarski Nicolas; Lamattina Lorenzo; Eguaras Martin

来源：Journal of Apicultural Research, 2014, 53(5): 576-589.

DOI：10.3896/IBRA.1.53.5.11

• Apis mellifera
• immunity
• haemocytes
• in-vitro

The honey bee Apis mellifera is affected by many parasites posing a serious threat to our ecosystem, agriculture and apiculture. The study of A. mellifera immune response represents a great complement for developing integrated disease management strategies. This work presents updated data concerning the in vitro cellular immune response of A. mellifera fifth instar larvae (L5) and newly emerged workers (W). Haemocytes from A. mellifera L5 and W clearly displayed different appearance in-vitro. The blood cells from these two developmental stages yielded behavioural differences regarding attachment and spreading. However, haemocytes extracted from L5 and W exhibited strong similarities with respect to their general (multicellular) behavioural pattern. This multi-haemolytic process, triggered after glass contact, could result from an encapsulation-like response. At the same time, an experimental system for studying A. mellifera cellular immunity in-vitro upon recognition of glass surfaces and haemolytic encapsulation response is herein developed.

• 出版日期2014