Association of cytokine gene polymorphisms and serum concentrations with the outcome of chronic hepatitis B

作者：Conde Simone R S; Feitosa Rosimar N M; Freitas Felipe Bonfim; Hermes Renata B; Demachki Samia; Araujo Marialva T F; Soares Manoel C P; Ishak Ricardo; Vallinoto Antonio C R^*

来源：Cytokine, 2013, 61(3): 940-944.

DOI：10.1016/j.cyto.2013.01.004

• Chronic hepatitis B
• Cytokine gene polymorphisms
• Cytokine serum levels

Objective: The present paper investigated possible correlations between the clinical presentation of hepatitis B and the TNF-alpha, -308G/A, IFN-gamma +874A/T, TGF-betal -509C/T, and IL-10 -1081A/G polymorphisms and associated serum levels of these cytokines.
Methods: Fifty-three hepatitis patients were selected and divided into two groups: A - inactive (n = 30) and B - chronic hepatitis/cirrhosis (n = 23). The control group consisted of 100 subjects who were positive for anti-HBc and anti-HBs. The serum concentrations of the cytokines were determined by immunoenzymatic assays. The polymorphisms of the cytokines genes were assessed by PCR and PCR-SSP.
Results: The mean serum levels of IFN-gamma of the control group were significantly higher than those of groups A and B, whereas the mean levels TGF-betal were significantly higher in groups A and B in comparison with the control. In the case of IL-10, the mean serum level recorded in the control group was significantly higher than that of group B. The TNF-alpha -308AG genotype was considerably more frequent in group B (43.3%) than the control (14.4%).
Conclusion: Higher serum levels of IFN-gamma and TGF-betal were associated with chronic hepatitis B, and lower serum levels of IL-10 were found in patients with the active disease. Furthermore the presence of allele A of the TNF-alpha -308 polymorphism suggest a risk of the progressive disease.

• 出版日期2013-3