Steady-state fluorescence quenching applications for studying protein structure and dynamics

作者：Matyus L^*; Szollosi J; Jenei A

来源：Journal of Photochemistry and Photobiology B: Biology , 2006, 83(3): 223-236.

DOI：10.1016/j.jphotobiol.2005.12.017

• fluorescence quenching
• protein structure
• FRET
• tryptophan fluorescence

Fluorescence quenching methods are useful to obtain information about the conformational and/or dynamic changes of proteins in complex macromolecular systems. In this review steady-state methods are described and the data interpretation is thoroughly discussed. As a special case of fluorescence quenching mechanism, fluorescence resonance energy transfer (FRET) phenomenon is also presented. Application of a FRET based method to characterize the temperature dependence of the flexibility of protein matrix is clearly demonstrated.

• 出版日期2006-6-1