Adsorption and Electrooxidation of DNA at Glassy Carbon Electrodes Modified with Multiwall Carbon Nanotubes Dispersed in Glucose Oxidase

作者：Gutierrez Fabiana; Rubianes Maria D^*; Rivas Gustavo A

来源：Electroanalysis, 2013, 25(5): 1135-1142.

DOI：10.1002/elan.201200559

• Carbon nanotubes dispersion
• Double stranded calf-thymus DNA
• Glucose oxidase
• Self-assembling multilayers
• Glassy carbon electrode
• DNA-drug interaction
• Methylene blue

We are proposing for the first time the successful immobilization of DNA at glassy carbon electrodes (GCE) modified with carbon nanotubes (CNT) dispersed in glucose oxidase (GOx) (GCE/CNT-GOx) either by direct adsorption or by layer-by-layer self-assembling using polydiallyldimethylamine (PDDA). The presence of GOx allows an efficient dispersion of CNT and gives a most favorable environment that promotes the adsorption and makes possible a more sensitive electrooxidation of DNA. The PDDA incorporated in the self-assembled architecture largely facilitates the adsorption and electrooxidation of dsDNA and the adsorbed layer can be successfully used for evaluating the interaction of DNA with methylene blue.

• 出版日期2013-5