Usefulness of Myoglobin Containing Cobalt Heme Cofactor in Designing a Myoglobin-Based Artificial Oxygen Carrier

作者：Neya Saburo^*; Yonetani Takashi; Kawaguchi Akira T

来源：Artificial Organs, 2014, 38(8): 715-719.

DOI：10.1111/aor.12327

• Myoglobin
• Metal replacement
• Cobalt heme
• Ligand binding
• Artificial oxygen carrier

The structure and reactivity of cobalt-replaced myoglobin (Mb) were investigated to explore its possible application as an artificial oxygen carrier. Ligand binding analysis with relaxation kinetics revealed that various ligands bind to Co(III) Mb, contrary to the earlier thoughts. The equilibration process, however, was so slow that it proceeded over 90 min. These characteristic profiles of oxidized Co(III) Mb were ascribed to the electronic structure of Co(III) ion which is one electron larger than Fe(III) ion. The oxygen affinity of reduced Co(II) Mb was much smaller than that of Fe(II) Mb indicating that Co(II) Mb has excellent oxygen transport ability. The latter observation, together with the lack of carbon monoxide binding in Co(II) Mb, suggests utility of Co(II) Mb as Mb-based oxygen carriers. The present results on cobalt-substituted Mb are useful in designing myoglobin-based oxygen carriers.

• 出版日期2014-8