A useful method integrating production and immobilization of recombinant cellulase

作者：Chiang Chung Jen; Chen Po Ting; Yeh Chien Yu; Wang Zei Wen; Chao Yun Peng^*

来源：Applied Microbiology and Biotechnology, 2013, 97(20): 9185-9192.

DOI：10.1007/s00253-013-5238-6

• Cellulase
• Artificial oil body
• Enzyme immobilization
• Response surface methodology

The development of cellulase-based bioprocess is afflicted by the processing efficiency of enzymes. To address this issue, a method based on artificial oil bodies (AOBs) was proposed to integrate production and immobilization of recombinant cellulase. First, the heterologous endoglucanase (celA), cellobiohydrolase (celK), and beta-glucosidase (gls) genes were individually fused with oleosin, a structural protein of plant seed oils. After expression in Escherichia coli, each fusion protein of insolubility was mixed together with plant oils. AOBs were assembled by subjecting the mixture to sonication. Consequently, active CelA, CelK, and Gls were resumed and co-immobilized on AOBs surface. Finally, the assembly condition (including the protein ratio) and the reaction condition were further optimized by response surface methodology. The resulting AOBs-bound cellulase remained stable for 4 cycles of cellulose-hydrolyzed reactions. Overall, the result shows a promise of this proposed approach for processing recombinant cellulase, which may provide a facile method to investigate optimum combination of cellulase components towards various cellulosic materials.

• 出版日期2013-10
• 单位中国医科大学