Prolonged Exposure to Lipopolysaccharide Induces NLRP3-Independent Maturation and Secretion of Interleukin (IL)-1 beta in Macrophages

作者：Hong Sujeong; Yu Je-Wook^*

来源：Journal of Microbiology and Biotechnology, 2018, 28(1): 115-121.

DOI：10.4014/jmb.1709.09017

• Inflammasome
• interleukin-1 beta
• lipopolysaccharide
• NLRP3

Upon sensing of microbial infections or endogenous danger signals in macrophages, inflammasome signaling plays a significant role in triggering inflammatory responses via producing interleukin (IL)-1 beta. Recent studies revealed that active caspase-1, a product of the inflammasome complex, causes maturation of inactive pro-IL-1 beta into the active form. However, the underlying mechanism by which this leaderless cytokine is secreted into the extracellular space remains to be elucidated. In this study, we demonstrated that prolonged lipopolysaccharide (LPS) treatment to macrophages could trigger the unexpected maturation and extracellular release of IL-1 beta through a nucleotide-binding oligomerization domain-like receptor family, pyrin domain-containing 3 (NLRP3)-independent manner. Short-term treatment (less than 6 h) of LPS induced robust production of the IL-1 beta precursor form inside cells but did not promote the maturation and secretion of IL-1 beta in bone marrow-derived macrophages or peritoneal macrophages. Instead, prolonged LPS treatment (more than 12 h) led to a significant release of matured IL-1 beta with no robust indication of caspase-1 activation. Intriguingly, this LPS-triggered secretion of IL-1 beta was also observed in NLRP3-deficient macrophages. In addition, this unexpected IL-1 beta release was only partially impaired by a caspase-1 and NLRP3 inflammasome inhibitor. Collectively, our results propose that prolonged exposure to LPS is able to drive the maturation and secretion of IL-1 beta in an NLRP3 inflammasome-independent manner.

• 出版日期2018-1