Identification of Tropilaelaps mites (Acari, Laelapidae) infesting Apis mellifera in China

作者：Luo Qi Hua; Zhou Ting^*; Wang Qiang; Dai Ping Li; Wu Yan Yan; Song Huai Lei

来源：Apidologie, 2011, 42(4): 485-498.

DOI：10.1007/s13592-011-0028-6

• Tropilaelaps mercedesae
• identification
• Apis mellifera
• mtDNA
• cox1
• ITS1-5.8S-ITS2
• China

Tropilaelaps mite samples were collected from 72 locations in 25 provinces throughout China. The mitochondrial DNA cox1 and ribosomal ITS1-5.8S-ITS2 fragments of Tropilaelaps mites were surveyed for sequence variation or the presence or absence of specific restriction sites that differentiate four species of Tropilaelaps mite (Tropilaelaps clareae, Tropilaelaps mercedesae, Tropilaelaps koenigerum, and Tropilaelaps thaii). Based on these identified diagnostic characters, all samples in this study corresponded to T. mercedesae, which has been mistaken for T. clareae until now. None of the other Tropilaelaps species were found infesting Apis mellifera in China. A total of 73 cox1 haplotypes and 104 ITS1-5.8S-ITS2 haplotypes were discovered in this study. Haplotype analysis revealed that there is no association between geographic distance and genetic distance among populations. The results of the present study clarified the taxonomic status and biogeography of Tropilaelaps mites in China, and should have implications for the control and bee quarantine efforts in China.

• 出版日期2011-8
• 单位中国农业科学院