A High-Throughput Multiplex Method Adapted for GMO Detection

作者：Chaouachi Maher; Chupeau Gaelle; Berapd Aurelie; McKhann Heather; Romaniuk Marcel; Giancola Sandra; Laval Valerie; Bertheau Yves; Brunel Dominique^*

来源：Journal of Agricultural and Food Chemistry, 2008, 56(24): 11596-11606.

DOI：10.1021/jf801482r

• SNPlex
• GMO
• detection
• multiplex
• high-throughput

A high-throughput multiplex assay for the detection of genetically modified organisms (GMO) was developed on the basis of the existing SNPlex method designed for SNP genotyping. This SNPlex assay allows the simultaneous detection of up to 48 short DNA sequences (similar to 70 bp; "signature sequences") from taxa endogenous reference genes, from GMO constructions, screening targets, construct-specific, and event-specific targets, and finally from donor organisms. This assay avoids certain shortcomings of multiplex PCR-based methods already in widespread use for GMO detection. The assay demonstrated high specificity and sensitivity. The results suggest that this assay is reliable, flexible, and cost- and time-effective for high-throughput GMO detection.

• 出版日期2008-12-24
• 单位中国地震局