Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and anti-inflammatory activities

作者：Kang Jie; Xie Chenghui; Li Zhimin; Nagarajan Shanmugam; Schauss Alexander G; Wu Tong^*; Wu Xianli

来源：Food Chemistry, 2011, 128(1): 152-157.

DOI：10.1016/j.foodchem.2011.03.011

• Acai
• Euterpe oleracea Mart.
• Flavonoid
• Antioxidant
• Anti-inflammation

Five flavonoids, (2S,3S)-dihyrokaempferol 3-O-beta-D-glucoside (1) and its isomer (2R,3R)-dihydrokaempferol 3-O-beta-D-glucoside (2), isovitexin (3), velutin (4) and 5,4'-dihydroxy-7,3',5'-trimethoxyflavone (5), were isolated from acai (Euterpe oleracea Mart.) pulp. The structures of these compounds were elucidated based upon spectroscopic and chemical analyses. To our knowledge, compounds 1, 2, 4 and 5 were identified from acai pulp for the first time. The in vitro antioxidant activities of these compounds were evaluated by the oxygen radial absorbance capacity (ORAC) assay. The ORAC values varied distinctly (4458.0-22404.5 mu mol Trolox equivalent (TE)/g) from 5,4'-dihydroxy-7,3',5'-trimethoxyflavone (5) to isovitexin (3) and were affected by the numbers/positions of hydroxyl groups, substitute groups, as well as stereo configuration. The anti-inflammatory effects of these compounds were screened by the secreted embryonic alkaline phosphatase (SEAP) reporter assay, which is designed to measure NF-kappa B activation. Velutin (4) was found to dose-dependently inhibit SEAP secretion in RAW-blue cells induced by LPS, with an IC(50) value of 2.0 mu M. Velutin (4) also inhibited SEAP secretion induced by oxidised LDL, indicating potential athero-protective effects.

• 出版日期2011-9-1
• 单位上海医药工业研究院