Neuropeptide Y is important for basal and seizure-induced precursor cell proliferation in the hippocampus

作者：Howell Owain W; Silva Sharmalene; Scharfman Helen E; Sosunov Alexander A; Zaben Malik; Shatya Anan; Mckhann Guy II; Herzog Herbert; Laskowski Alexandra; Gray William P^*

来源：Neurobiology of Disease, 2007, 26(1): 174-188.

DOI：10.1016/j.nbd.2006.12.014

• NPY
• neurogenesis
• progenitor cell
• stem cell
• epilepsy
• cell culture
• nestin
• subventricular zone
• subcallosal zone
• dentate gyrus

We have shown that neuropeptide Y (NPY) regulates neurogenesis in the normal dentate gyrus (DG) via Y-1 receptors (Howell, O.W., Scharfman, H.E., Herzog, H., Sundstrom, L.E., Beck-Sickinger, A. and Gray, W.P. (2003) Neuropeptide Y is neuroproliferative for post-natal hippocampal precursor cells. J Neurochem, 86, 646-659; Howell, O.W., Doyle, K., Goodman, J.H., Scharfman, H.E., Herzog, H., Pringle, A., Beck-Sickinger, A.G. and Gray, W.P. (2005) Neuropeptide Y stimulates neuronal precursor proliferation in the post-natal and adult dentate gyrus. J Neurochem, 93, 560-570). This regulation may be relevant to epilepsy, because seizures increase both NPY expression and precursor cell proliferation in the DG. Therefore, the effects of NPY on DG precursors were evaluated in normal conditions and after status epilepticus. In addition, potentially distinct NPY-responsive precursors were identified, and an analysis performed not only of the DG, but also the caudal subventricular zone (cSVZ) and subcallosal zone (SCZ) where seizures modulate glial precursors. We show a proliferative effect of NPY on multipotent nestin cells expressing the stem cell marker Lewis-X from both the DG and the cSVZ/SCZ in vitro. We confirm an effect on proliferation in the cSVZ/SCZ of Y-1 receptort(-/-) mice and demonstrate a significant reduction in basal and seizure-induced proliferation in the DG of NPY-/- mice.

• 出版日期2007-4
• 单位河北医科大学