Antimelanomic Effects of High-and Low-Molecular Weight Bioactive Subfractions Isolated from the Mossy Maze Mushroom, Cerrena unicolor (Agaricomycetes)

作者：Statkiewicz Malgorzata; Matuszewska Anna; Jaszek Magdalena; Janusz Grzegorz; Osinska Monika; Sulej Justyna; Stefaniuk Dawid; Mikula Michal; Ostrowski Jerzy

来源：International Journal of Medicinal Mushrooms, 2017, 19(7): 619-628.

DOI：10.1615/IntJMedMushrooms.2017021241

• antimelanomic effect
• endopolysaccharides
• immunostimulation
• laccase
• low-molecular weight compounds
• medicinal mushrooms
• white rot fungi

Three bioactive fractions isolated from Cerrena unicolor cultures-crude endopolysaccharide (c-EPS), laccase, and a subfraction of low-molecular weight secondary metabolites-were used to determine potential cytotoxic effects on the mouse melanoma B16-F10 cell line (American Type Culture Collection CRL-6475). The results obtained prove that all examined fractions exhibited activity against the investigated tumor cells. In addition, an evident immunomodulatory effect of the c-EPS fraction was observed. Our results show that the levels of 2 cytokines (tumor necrosis factor-alpha and chemokine ligand 2) in mouse inner medullary collecting duct mIMCD-3 cells (American Type Culture Collection CRL-2123) stimulated by c-EPS were significantly higher. A lipopolysaccharide model was used at the same concentration (10 mu g/mL) as a positive control.

• 出版日期2017