Low-dose diethylhexyl phthalate exposure does not impair the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in mouse mammary glands

作者：Cheng, Shun-Feng; Li, Ling; Li, Bo; Liu, Jing-Cai; Lai, Fang-Nong; Zhao, Yong; Zhang, Xi-Feng; Shen, Wei; Li, Lan

来源：Molecular & Cellular Toxicology, 2018, 14(2): 143-153.

DOI：10.1007/s13273-018-0016-2

• DEHP
• Mammary gland cells
• Epigenetics
• DNA methylation

Backgrounds: Di-(2-ethylhexyl) phthalate (DEHP), as an endocrine-disrupting chemical (EDC), is widely used in plasticizer and other productions. Ubiquitous human exposure to DEHP has been proposed to be a potential risk to public health. Developmental exposure to DEHP could alter epigenetic programming and result in adult-onset disease. @@@ Methods: In this study, we investigated whether DEHP exposure to pregnant mice affected epigenetic changes as a result of increase in breast cancer incidence. @@@ Results: Our results showed that the expression of total 143 epigenetics-related genes in mammary gland cells, have no significantly altered after short time and low-dose treated with DEHP from 0.5 days post-coitum (dpc) to 3.5 dpc of pregnant mice. DNA methylation status of some neoplastic development genes, such as EGFr, Esr1, Pgr, Fos and Rassf5 also had no obvious change. @@@ Conclusion: These finding showed no impact of DEHP on the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in pregnant mouse mammary gland cells.

• 出版日期2018-4
• 单位青岛农业大学; 武汉轻工大学