A novel real-time PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax malaria in low parasitized individuals

作者：Hwang Seung Young; Kim So Hee; Lee Ga Young; Vu Thi Thu Hang; Moon Chi Sook; Shin Jeong Hwan; Koo Wan Lim; Kim Seong Youl; Park Hae Joon; Park Han Oh; Kho Weon Gyu^*

来源：Acta Tropica, 2011, 120(1-2): 40-45.

DOI：10.1016/j.actatropica.2011.05.006

• Malaria
• Diagnosis
• Real-time PCR
• Low parasitemia

The rapid, accurate diagnosis of Plasmodium spp. is essential for the effective control of malaria, especially in asymptomatic infections. In this study, we developed a sensitive, genus-specific, real-time quantitative PCR assay. It was compared with the microscopic examination of Giemsa-stained blood smears and two different molecular diagnostic techniques: nested PCR and multiplex PCR. For the effective quantitative detection of malaria parasites, all reagents were designed with a lyophilized format in one tube. Plasmodium was detected successfully in all 112 clinically suspected malaria patients, including 32 individuals with low parasitemia (1-100 parasites/mu l). The sensitivity threshold was 0.2 parasites/mu l and no PCR-positive reaction occurred when malaria parasites were not present. This may be a useful method for detecting malaria parasites in endemic areas.

• 出版日期2011-11