Molecular cloning, characterization and expression analysis of a C-type lectin (AJCTL) from the sea cucumber Apostichopus japonicus

作者：Han, Lu-Lu; Yuan, Zhe; Dahms, Hans-Uwe; Li, Qiu-Ying; Zhang, Qiao-Zhi; Wu, Rong-Jie; Tan, Jian; Zou, Xiang-Yang; Hou, Lin^*

来源：Immunology Letters, 2012, 143(2): 137-145.

DOI：10.1016/j.imlet.2011.12.004

• Sea cucumber
• Apostichopus japonicus
• C-type lectin
• Cloning
• Tissue distribution
• mRNA expression
• RT-PCR

For the sea cucumber Apostichopus japonicus, a C-type lectin(AJCTL) was identified using rapid amplification of cDNA ends (RACE) PCR techniques. The full-length cDNA of AJCTL is composed of 710 bp with a 618 bp open reading frame(ORF) that encodes a polypeptide of 205 amino acids with a N-terminal signal peptide and a C-terminal C-type lectin domain (CTLD). The calculated molecular mass of the whole protein is 22.5 kDa and its predicted isoelectric point is 5.59. AJCTL belongs to the group VII of regulatory proteins and it might function as a Ca2+-dependent monosaccharide binding lectin specifically and recognizing mannose-type ligands. In situ hybridization demonstrated that the expression of AJCTL was located in the body wall, longitudinal muscles, intestinum and respiratory tree. This became apparent especially in the cytoplasm of epidermal cells and granular haemocytes. Real-time PCR data suggested that AJCTL was mostly synthesized in the longitudinal muscles and intestinum and less pronounced in the respiratory tree and body wall of adults. After 12 h stimulation by Vibrio harveyi, at increasing bacterial concentration gradient, the expression of AJCTL in sea cucumber increased as well. This indicated that CTL is related to an innate immune response.

• 出版日期2012-4-30
• 单位大连医科大学; 辽宁师范大学