Reduction of urinary uric acid excretion in patients with proteinuria

作者：Zou Huiqing; Xiang Mingfeng; Ye Xinming; Xiong Yuanzhen; Xie Baogang^*; Shao Jianghua

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2015, 1006: 59-64.

DOI：10.1016/j.jchromb.2015.10.027

• Proteinuria
• HPLC-HILIC-UV
• Uric acid
• Xanthine
• Hypoxanthine and creatinine

Serum uric acid (UA) concentration is positively associated with proteinuria. However, the relationship between proteinuria and urinary metabolites of purine metabolism remains unknown. This study developed a hydrophilic interaction chromatography (HILIC)-based HPLC method with ultraviolet detection (UV) to quantify creatinine (Cr), UA, xanthine, and hypoxanthine in human urine simultaneously. The urinary concentrations of UA and Cr obtained by our method are consistent with those measured by an autoanalyzer. The HPLC-HILIC-UV method was validated as selective and robust with simple sample preparation for measuring UA, xanthine, hypoxanthine and Cr, which is suitable for large clinical studies. The UA/Cr ratios in random urine samples were 5.5 times lower in proteinuria patients (0.077 +/- 0.008) than in healthy individuals (0.424 +/- 0.037). Moreover, the UA/hypoxanthine ratio in proteinuria patients was approximately 10 times lower than that in healthy individuals. Our findings revealed a reduced urinary UA excretion, which is one of the factors leading to increased serum UA in proteinuria patients.

• 出版日期2015-12-1
• 单位南昌大学