Preparation of a Cross-Linked Porous Protein Crystal Containing Ru Carbonyl Complexes as a CO-Releasing Extracellular Scaffold

作者：Tabe Hiroyasu; Fujita Kenta; Abe Satoshi; Tsujimoto Masahiko; Kuchimaru Takahiro; Kizaka Kondoh Shinae; Takano Mikio; Kitagawa Susumu^*; Ueno Takafumi

来源：Inorganic Chemistry, 2015, 54(1): 215-220.

DOI：10.1021/ic502159x

Protein crystals generally are stable solid protein assemblies. Certain protein crystals are suitable for use as nanovessels for immobilizing metal complexes. Here we report the preparation of ruthenium carbonyl-incorporated cross-linked hen egg white lysozyme crystals (Ru center dot CL-HEWL). Ru center dot CL-HEWL retains a Ru carbonyl moiety that can release CO, although a composite of Ru carbonyl-HEWL dissolved in buffer solution (Ru center dot HEWL) does not release CO. We found that treatment of cells with Ru center dot CL-HEWL significantly increased nuclear factor kappa B (NF-kappa B) activity as a cellular response to CO. These results demonstrate that Ru center dot CL-HEWL has potential for use as an artificial extracellular scaffold suitable for transport and release of a gas molecule.

• 出版日期2015-1-5