AC3-33, a novel secretory protein, inhibits Elk1 transcriptional activity via ERK pathway

作者：Hao Dongxia; Gao Peng; Liu Peng; Zhao Jie; Wang Yang; Yang Wenping; Lu Yang; Shi Taiping; Zhang Xiujun^*

来源：Molecular Biology Reports, 2011, 38(2): 1375-1382.

DOI：10.1007/s11033-010-0240-x

• AC3-33
• Secretory protein
• Inhibit
• Elk1
• ERK pathway

The transcription factor AP-1 plays an important role in cellular proliferation, transformation and death. In this study, we report a novel human gene, AC3-33 (GenBank name: c3orf33, FLJ31139), which encodes a secretory protein that can inhibit Elk1 transcriptional activity via ERK1/2 pathway. The AC3-33 mRNA encodes a protein of 251 amino acids, which is a classical secretory protein. Functional investigation reveals that overexpression of AC3-33 significantly inhibit AP-1 activity and DNA-binding ability. Further investigation indicated that overexpression of AC3-33 significantly inhibit transcriptional activity of Elk1 and c-jun, but not c-fos. As for the upstream of signaling pathway of Elk-1, our study demonstrated that overexpression of AC3-33 significantly down-regulates phosphorylation of ERK1/2, but not JNK/SAPK or p38 MAPK. These results clearly indicate that AC3-33 is a novel member of the secretory family and inhibits Elk1 transcriptional activity via ERK1/2 MAPK.

• 出版日期2011-2
• 单位国家卫生计生委科学技术研究所; 华北理工大学