Analysis of vaginal lactic acid producing bacteria in healthy women

作者：Nam Hyeran; Whang Kyunghee; Lee Yeonhee^*

来源：Journal of Microbiology, 2007, 45(6): 515-520.

• vaginal microbiota
• lactic acid-producing bacteria
• denaturing gradient gel electrophoresis
• temporal temperature gradient gel electrophoresis

Vaginal lactic acid-producing bacteria of 80 pre-menopausal women were studied by isolation on Blood and DeMan-Rogosa-Sharpe agar, PCR with group-specific primers for Lactobacillus-denaturing gradient gel electrophoresis (DGGE), and PCR with specific primers for V3 region in 16S rRNA-temporal temperature gel electrophoresis (TTGE). Conventional isolation method on media detected only one lactobacillus (Lactobacillus brevis) while TTGE detected only Lactobacillus sp. DGGE detected seven Lactobacillus species; L. coleohominis, L. crispatus, L. iners, L reuteri, L. rhamnosus, L. vaginalis, and Leuconostoc lactis. L acidophilus and L. gasseri, which are prevalent in Western women, were not detected in Korean women. Furthermore, L. rhamnosus, Leuc. lactis, L. coleohominis, and Weissella cibaria, which were not previously reported in the vaginal microbiota of Korean women, were detected. The five most prevalent LABs in vaginal microbiota in Korean women were L iners, Enterococcus faecalis, L. crispatus, Leuc. lactis, and W. cibaria.

• 出版日期2007-12