A simple and selective method for determination of phthalate biomarkers in vegetable samples by high pressure liquid chromatography-electrospray ionization-tandem mass spectrometry

作者：Zhou, Xi; Cui, Kunyan; Zeng, Feng^*; Li, Shoucong; Zeng, Zunxiang

来源：Food Chemistry, 2016, 200: 336-342.

DOI：10.1016/j.foodchem.2016.01.008

• Phthalate monoester metabolites
• Ultrasonic extraction
• Liquid-liquid extraction
• Solid-phase extraction
• Vegetable
• High pressure liquid chromatographyelectrospray ionisation-tandem mass spectrometry

In the present study, solid-phase extraction cartridges including silica reversed-phase Isolute C-18, polymeric reversed-phase Oasis HLB and mixed-mode anion-exchange Oasis MAX, and liquid-liquid extractions with ethyl acetate, n-hexane, dichloromethane and its mixtures were compared for clean-up of phthalate monoesters from vegetable samples. Best recoveries and minimised matrix effects were achieved using ethyl acetate/n-hexane liquid-liquid extraction for these target compounds. A simple and selective method, based on sample preparation by ultrasonic extraction and liquid-liquid extraction clean-up, for the determination of phthalate monoesters in vegetable samples by liquid chromatography/electrospray ionisation-tandem mass spectrometry was developed. The method detection limits for phthalate monoesters ranged from 0.013 to 0.120 ng g (1). Good linearity (r(2) > 0.991) between MQLs and 1000 x MQLs was achieved. The intra-and inter-day relative standard deviation values were less than 11.8%. The method was successfully used to determine phthalate monoester metabolites in the vegetable samples.

• 出版日期2016-6-1
• 单位中山大学