科研之友
微信
新浪微博
Facebook
分享链接
摘要
More and more researchers pay attention to the response of endothelial cells during tumor radiotherapy, and most researches presented that endothelial cell apoptosis ratio influences the efficacy of radiotherapy. Nevertheless, radiation-induced gene conversions in human cervical endothelial cells (HCECs) remain rarely understood. This study would address the gene expression conversions after X-ray radiation in these cells and provide novel treatment strategies for human cervical cancer. We obtained endothelial cells from six human cervical cancer patients before and 4 h after 400 cGy X-ray irradiation. The significant genes were selected by a 22K Human Genome Oligonucleotide Microarray Kit and confirmed by real-time quantitative PCR and western blot. Our results showed that 46 genes consistently altered in all the microarray experiments. Compared with the control group, 26 genes were up-regulated and 20 genes were down-regulated. Most of the genes were identified as significant conversions (>= 2 fold), including the regulation of the cell cycle, cell adhesion, the immune response, chemokines, the inflammatory response, growth factors, angiogenesis, DNA synthesis and repair, and protein synthesis. Four genes were randomly selected to confirm the microarray data. Radiation-induced gene alterations and gene-related signal pathways were related to vasculogenesis and radiosensitivity of human cervical cancer. This study elucidates that the identified genes and gene-related signaling pathways may provide meaningful biomarkers for radiation and anti-angiogenesis treatments against human cervical cancer.
