A Novel Non-canonical Forkhead-associated (FHA) Domain-binding Interface Mediates the Interaction between Rad53 and Dbf4 Proteins

作者：Matthews Lindsay A; Selvaratnam Rajeevan; Jones Darryl R; Akimoto Madoka; McConkey Brendan J; Melacini Giuseppe; Duncker Bernard P; Guarne Alba^*

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289(5): 2589-2599.

DOI：10.1074/jbc.M113.517060

• Bioinformatics
• Checkpoint Control
• DNA Damage Response
• DNA Replication
• NMR
• Protein Domains
• Protein-Protein Interactions
• Yeast Genetics

Background: The interaction between the checkpoint kinase Rad53 and Dbf4 is critical to suppress late origin firing and to stabilize stalled forks during replication stress. Results: The FHA1 domain of Rad53 interacts with the BRCT domain of Dbf4 through a novel non-canonical interface. Conclusion: FHA domains gain specificity by engaging multiple binding interfaces. Significance: Understanding how FHA domains interact with their binding partners is key to elucidate how they relay information along signaling pathways.
Forkhead-associated (FHA) and BRCA1 C-terminal (BRCT) domains are overrepresented in DNA damage and replication stress response proteins. They function primarily as phosphoepitope recognition modules but can also mediate non-canonical interactions. The latter are rare, and only a few have been studied at a molecular level. We have identified a crucial non-canonical interaction between the N-terminal FHA1 domain of the checkpoint effector kinase Rad53 and the BRCT domain of the regulatory subunit of the Dbf4-dependent kinase that is critical to suppress late origin firing and to stabilize stalled forks during replication stress. The Rad53-Dbf4 interaction is phosphorylation-independent and involves a novel non-canonical interface on the FHA1 domain. Mutations within this surface result in hypersensitivity to genotoxic stress. Importantly, this surface is not conserved in the FHA2 domain of Rad53, suggesting that the FHA domains of Rad53 gain specificity by engaging additional interaction interfaces beyond their phosphoepitope-binding site. In general, our results point to FHA domains functioning as complex logic gates rather than mere phosphoepitope-targeting modules.

• 出版日期2014-1-31