Direct Derivatization vs Aqueous Extraction Methods of Fecal Free Fatty Acids for GC-MS Analysis

作者：Amer Bashar; Nebel Caroline; Bertram Hanne C; Mortensen Grith; Dalsgaard Trine K^*

来源：Lipids, 2015, 50(7): 681-689.

DOI：10.1007/s11745-015-4029-5

• Targeted GC-MS metabolomics
• Fecal free fatty acid
• Ethyl chloroformate derivatization

A comprehensive and accurate determination of free fatty acids (FFA) is required for fecal metabolomic investigations. The present study compares three aqueous extraction methods (1) ULTRA-TURRAX(A (R)), (2) whirl mixing and (3) basic ULTRA-TURRAX extraction of fecal FFA with a direct derivatization approach using ethyl chloroformate as the derivatization reagent before determination by gas chromatography-mass spectrometry. The direct derivatization method resulted in significantly higher estimations (P < 0.01) of short- and long-chain fatty acids than was the case when applying the aqueous extraction methods using ULTRA-TURRAX, whirl mixing, or basic ULTRA-TURRAX extraction before the derivatization step. Thus, avoiding an aqueous extraction before derivatization reduces the loss of volatile short-chain FFA and the less water-soluble long-chain FFA.

• 出版日期2015-7