Development of a Multiplex Sandwich Aptamer Microarray for the Detection of VEGF(165) and Thrombin

作者：Sosic Alice; Meneghello Anna; Antognoli Agnese; Cretaio Erica; Gatto Barbara^*

来源：Sensors, 2013, 13(10): 13425-13438.

DOI：10.3390/s131013425

• VEGF
• thrombin
• aptamers
• sandwich aptamer-microarray
• angiogenesis

In this work we have developed a multiplex microarray system capable of detecting VEGF(165) and thrombin. We recently described a Sandwich Aptamer Microarray (SAM) for thrombin detection feasible for use in multiplex microarrays; here we describe a new aptasensor for VEGF(165) detection employing Vap7 and VEa5, two DNA aptamers recognizing different sites of the protein. The aptamers were modified to be adapted to the solid phase platform of SAM and their capability to simultaneously recognize VEGF(165) by forming a ternary complex was analyzed in solution. Having so defined the best tandem arrangement of modified aptamers, we set up the aptasensor for VEGF(165), and finally analyzed the multiplex system with the two aptasensors for the simultaneous detection of VEGF(165) and thrombin. The results indicate that each sandwich is specific, even when the two proteins are mixed. The system performance is consistent with the behavior evidenced by the biochemical analysis, which proves to be valuable to drive the evaluation and refinement of aptamers prior to or along the development of a detection platform. Since thrombin upregulates VEGF expression, the simultaneous recognition of these two proteins could be useful in the analysis of biomarkers in pathologies characterized by neo-angiogenesis.

• 出版日期2013-10