Antiangiogenic and antitumor effects of IMMUNEPOTENT CRP in murine melanoma

作者：Franco Molina Moises A^*; Mendoza Gamboa Edgar; Zapata Benavides Pablo; Castillo Tello Paloma; Isaza Brando Clara E; Zamora Avila Diana; Rivera Morales Lydia G; Miranda Hernandez Diana F; Sierra Rivera Crystel A; Vera Garcia Magda E; Tamez Guerra Reyes S; Rodriguez Padilla Cristina

来源：Immunopharmacology and Immunotoxicology, 2010, 32(4): 637-646.

DOI：10.3109/08923971003663253

• Apoptosis
• bovine dialyzable leukocyte extract
• IMMUNEPOTENT CRP
• melanoma tumor
• VEGF

Background: Skin cancers are common, and there has recently been a dramatic increase in their incidence, particularly in the occurrence of melanoma. Furthermore, relapse after curative surgical treatment of melanoma remains a significant clinical challenge and accounts for most of the mortality of this disease. Objective: The aim of this study was to determine whether IMMUNEPOTENT CRP affects B16F10 melanoma cells and tumors growth and vascular endothelial growth factor (VEGF) production in vivo and in vitro.
Methods: B16F10 cells and B16F10-inoculated mice were treated with different concentrations of IMMUNEPOTENT CRP. Outcomes were then evaluated using MTT, TUNEL, Caspase-3, senescence, ELISA and colorimetric assays. Parameters related to survival and tumor weight were also assessed.
Results: IMMUNEPOTENT CRP decreased the viability of B16F10 cells by increasing apoptosis of the treated cells, and VEGF production was decreased both in vitro and in vivo. Furthermore, treatment prevented metastasis, delayed the appearance of tumors, decreased tumor weight and improved the survival of tumor-bearing mice.
Discussion: These observations suggest that IMMUNEPOTENT CRP can be used to suppress growth and metastasis by using targeting proteins such as VEGF.

• 出版日期2010-12