Norditerpenoids from Flickingeria fimbriata and Their Inhibitory Activities on Nitric Oxide and Tumor Necrosis Factor-alpha Production in Mouse Macrophages

作者：Chen, Jin Long; Zhong, Wen Jun; Tang, Gui Hua; Li, Jing; Zhao, Zhi Min; Yang, De Po^*; Jiang, Lin

来源：Molecules, 2014, 19(5): 5863-5875.

DOI：10.3390/molecules19055863

• norditerpenoid
• TDDFT
• exiton chirality method
• NO
• TNF-alpha

Bioassay-guided fractionation of the ethanolic extract of the leaves of Flickingeria flimbriata led to the isolation of two new degraded diterpenoids 1 and 2, a new ent-pimarane type diterpenoid 3, and four known steroids 4-7. The structures of 1-3 were elucidated by spectroscopic analysis, and their absolute configurations were determined by chemical methods, TDDFT quantum chemical calculations of ECD spectra, and CD exiton chirality method. Compounds 1 and 2, named flickinflimilins A and B, possess a rare 15,16-dinor-ent-pimarane skeleton. Compounds 1-7 were screened for the inhibitory activity against lipopolysaccharide (LPS)-induced NO and TNF-alpha production in RAW264.7 cells. Compounds 1-3 exhibited potent inhibitory activities, with IC50 values of less than 10 mu M.

• 出版日期2014-5
• 单位中山大学