科研之友
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摘要
The bacteriocin release protein (BRP) mediated release of several eukaryotic proteins was studied in Escherichia coli. The genes for the plant proteins a-galactosidase and thaumatin, and for the mammalian proteins prochymosine and prophospholipase A(2), were cloned behind the OmpA signal peptide. The alpha-galactosidase was expressed and secreted into the periplasm. Prochymosine and thaumatin were poorly expressed. Release experiments showed that about 5 mg/l of alpha-galactosidase was excreted into the extracellular medium upon induction of the BRP.
- 出版日期1995-8
